Berliana Naomi Rumondang Sari Aritonang

Akademi Kesehatan John Paul II Pekanbaru

e-mail : berliana.aritonang@gmail.com

Jurnal Sains dan Teknologi Laboratorium Medik. ISSN: 2527-5267.

Vol.1. No.1 (2016) 44-59


ABSTRAK

Penggunaan pestisida kimiawi dikalangan pertanian dapat menimbulkan berbagai macam masalah pada bidang kesehatan oleh karena itu para ahli telah menemukan bahan alternatif berupa pestisida alami yaitu biofungisida. Pengaruh kontaminasi penggunaan biofungisida yang mengandung spora Trichoderma sp terhadap bidang kesehatan belum diketahui. Biofungisida yang mengandung Trichoderma sp yang menghasilkan antibiotika berupa gliotoxin dan viridian dimana zat tersebut bersifat toksik dan dapat terakumulasi di dalam tubuh manusia maupun hewan. Tujuan penelitian ini untuk mengetahui toksisita biofungisida terhadap gambaran histopatologi testis. Penelitian ini menggunakan hewan uji mencit albino (Mus musculus) berumur 12 minggu, dan dibagi menjadi 5 kelompok, masing – masing kelompok terdiri dari 5 ekor mncit. Pemberian biofungisida dilakukan secara oral dengan dosis 0 cc/Kg BB, 16 cc/Kg BB, 24 cc/Kg BB, 36 cc/Kg BB, dan 54 cc/Kg BB setiap hari selama 14 hari. Data dianalisis dengan ANOVA dan dilanjutkan dengan uji lanjut Bonferroni dengan taraf signifikan p<0,05. Hasil penelitian menunjukkan penurunan berat testis, diameter tubulus seminiferous, tebal epitel germinal tubulus seminiferous, diameter inti sel leydig, jumlah sel leydig, dan jumlah sel sertoli terjadi perbedaan bermakna antara kelompok control dengan kelompok perlakuan. Untuk indeks spermatogenesis kelompok control menunjukkan oenurunan terbesar pada dosis 54 cc/Kg BB sebesar 66,032%. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian biofungisida mempengaruhi berat berat testis dan histopatologi testis, jadi biofungisida tersebut bersifat toksik terhadap organ reproduksi mencit jantan albino (Mus musculus).
Kata kunci : biofungisida, testis, trichoderma sp

ABSTRACT

Due to the fact that the use of pesticides in agriculture has potential health effects, expert have invented a variable alternative like biofungicides. However, the potential risk of biofungicides contamination on human health is still unknown. Biofungicide containing Trichoderma sp spore produce antibiotic like gliotoxin and viridian which could be toxic, and they could accumulate over time in the bodies of human and animal. The purpose of this study was to measure the toxicity of biofungicide on histopathology of male reproductive system. The study was an experimental study which used tweleve-week-old male albino laboratory mice (Mus musculus). Those mice were divided into five groups. Each group consisted of five mice. Biofungicide were orally administered in the dose 0, 16, 24, 36, and 54 cc per kilogram body weight daily for 14 days. The data were analyzed using Analysis of Variance and Bonferoni test, difference was considered significant when P value was < 0,05. The result showed a decrease in testis weight, diameter of seminiferous tubules, nuclear diameter of leydig cell, the number of leydig cell, and the number of certoly cell. There were significant differences between control groups and treatment groups. The comparison of average spermatogenic index showed significant difference between control group and treatment group; the greatest decrease (66,032%) was found in the dose 54 cc per kilogram body weight. In conclusion, biofungicide affects the testis weight and histopathology of testes. Consequently, it is toxic to the male reproductive system of albino laboratory mice (Mus Musculus).
Keywords : biofungicide, testes, trichoderma sp

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